Penetapan Keragaman Genetik Nilam (Pogostemon SP.) Hasil Fusi Protoplas Dengan Teknik Rapd. Yang Nuryani • Otih Rostiana • Cheppy Syukur. Pengembangan Metode Fusi Protoplas dengan Tingkat Keberhasilan Pembentukan Fusan 25% untuk Memperoleh Tanaman Triploid Jeruk Garut Tanpa Biji. Request PDF on ResearchGate | Karakterisasi Dan Identifikasi Molekuler Fusan Hasil Fusi Protoplas Interspesies Chlorella pyrenoidosa dan.

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Result showed that Java patchouli Girilaya contained phenols and lignin respectively Email this article Login required.

Abstract Evaluation of phenols and lignin in the root of hybrid patchouli Pogostemon cablin Benth. Download full text Bahasa Indonesia, 6 pages. The strategy chosen to assemble the triploid tangerine because the technique of protoplast lrotoplas, fusion and regeneration of tangerine with diploid level has been mastered ploidy. User Username Password Remember me. S Assessment of genetic variability of patchoulli Pogostemon sp.

Fusi protoplas interspesifik aspergillus Spp

Pre cold treatment for more than 3 days on the response forming callus from pretreatment less vusi 3 days. It has been found thai Ihe plain resistance lo nematode is closely related to phenols and lignin content of the root Therefore. Seedless citrus improvement can be done by manipulating the level ploidy.

Haploid cell population repeatedly sub cultured every 4 weeks in a double layer media: DNA of the plant materials used were extracted by using the modified method of orozcocastulo et al. The second group was then classified into 2 minor groups. Some features of this site may not work without it. In the fusion of Girilaya x Sidikalang.


Ihe level of resistance of different clones of somatic hybrids lo nematode was approached by evaluating ihe content of phenols and lignin of the roots The study progoplas conducted al the laboratory of protoolas Research Institute for food Crop Biotechnology, Bogor in November Article Tools Print this article.

How to cite item. Evaluation of phenols and lignin in the root of hybrid patchouli Pogostemon cablin Benth. Email the author Login required. Language Select Language English Indonesian.

Sidikalang and TT 75 were tested for their genetic variability and relationship. Somatic hybridization by fusion technique is a breakthrough technique that allows the plant generated by an increased level ploidy. OPH 19 and Abi Ihe somatic hybrids protop,as be categorized in three group The irst groups 5 clones contained high phenols higher than the average. Five random primers, OPD Improvement of the plant resistance to nematode in patchouli is dificult 10 be conducted through conventional method as the plant never llowcrs.

Ihe second group 9 clones contained high lignin.

This orange tastes sweet but relatively many seeds fruit seed. Industrial Crops Research Journal. Abstract Tangerine is a citrus seed Indonesia and including a true species of the genus Citrus. Jurnal Penelitian Tanaman Industri merupakan publikasi ilmiah primer yang memuat hasil penelitian Pusat Penelitian dan Pengembangan Perkebunan Jln. Plants with triploid ploidy level usually have sterile fruit seedless.

Penetapan Keragaman Genetik Nilam (Pogostemon SP.) Hasil Fusi Protoplas Dengan Teknik Rapd – Neliti

The best enzyme formulations for the isolation of protoplasts from diploid callus and leaves in vitro and protoplast Fisi pollen is macerozim 0. View original from ejurnal.


Metadata Show full item record. One of the problems faced in patchouli production is nematode infection Pratylenchus brachyurus. The plant material used for the production of diploid callus is a population of diploid cells induced from embryonic nucellar tangerine.

Prosiding KPSDA

First group consisted of somatic hybrids nos. Based on the distribution of phenols and lignin content.

The genetic relationship among the somatic hybrids were estimated by using the index of similarity to perform genctical matrix and dendogram. Results showed that index of similarities of the amplified DNA from 5 random primers ranged from 0. Phenol and lignin content in the somatic hybrids were dilferenl in Ihe respective pairs.

Penetapan Keragaman Genetik Nilam (Pogostemon SP.) Hasil Fusi Protoplas Dengan Teknik Rapd

Index of similarity among genotypes were calculated by using NTsys ver. JavaScript is disabled for your browser. The somatic hybrids and their parental plants subjected to RAPD analyses were classified into 2 major groups, first, the parental group of Java patchouli and second, others parental plants, Aceh patchouli Sidikalang and TT 75and the somatic hybrids.

Meanwhile, from Ihe fusion of Girilaya, Tapak Tuan.